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Objective: To study cutaneous leishmaniasis (CL) for identifying the dominant Leishmania\udspecies on CL patients referred to medical health centers of historical Kashan city and suburbs\udlocated in Isfahan province in central part of Iran during 2010 to 2011. Methods: From 137 CL\udcases, were microscopically positive, the skin lesion serosity materials of 103 cases were cultured\udin monophasic culture media (RPMI 1 640). We used the PCR-RFLP method for characterization\udthe Leishmania isolates, by using specific internal transcribed spacer (ITS1) primers and HAEШ\udas the restriction fast enzyme. DNA was extracted from 63 samples. Results: L. tropica is main\udspecies in 58 (92.1%) cases and L. major is identified in 5 (7.9%) cases. Indeed randomly two\udisolates were the species characterized as L. major produced ulcer at the base tail of BALB/c mice\udafter 3 weeks but from three L. tropica isolates none of them produced any lesion during 6 months\udpost inoculation. Conclusions: The parasitological, epidemiological aspect and molecular\udmethods of this study showed that, Kashan and suburb are anthroponetic CL area despite this city\udlocated in Isfahan province as an ancient focus of zoonotic CL in Iran

A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran