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Objective: To assess the phytochemical constituents, total phenolic content, cytotoxicity and invitro antioxidant activity of stem bark extracts of Moringa oleifera (M. oleifera) (Moringaceae).\udMethods: Brine shrimp lethality (BSL) bioassay was used to investigate the cytotoxic effects.\udDPPH and nitric oxide radical scavenging activity was used to demonstrate antioxidant activity.\udResults: Phytochemical analysis revealed the presence of tannins, flavonoids, steroids and\udalkaloids. The LC\ud50 values were obtained for extracts as 850 毺 g/mL for petroleum ether extract, 800\ud毺 g/mL for chloroform extract and 900 毺 g/mL for methanol extract. The total phenolic content of\udthe methanolic extract was 50.72% w/w, equivalent to gallic acid. Petroleum ether, chloroform and\udmethanolic extracts of M. oleifera and standard ascorbic acid were found to be scavenger of DPPH\udradical with an IC\ud50 of 124.75, 112.08, 54.34 and 13.86 毺 g/mL, respectively. Methanolic extract was\udfound to be good scavenger of DPPH radical. Petroleum ether, chloroform, ethyl acetate soluble\udfraction of methanolic extracts of M. oleifera and ascorbic acid were found to be scavenger of\udnitric oxide radical with an IC\ud50 of 93.32, 65.12, 54.83 and 12.59 毺 g/mL, respectively. Ethyl acetate\udsoluble fraction was found to be good scavenger of nitric oxide radical. Conclusions: It can be\udconcluded that the crude extracts of M. oleifera is a potential source of natural antioxidants, and\udthis justifies its uses in folkloric medicines

Estimation of total phenolic content, cytotoxicity and in–vitro antioxidant activity of stem bark of Moringa oleifera