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mice) as well as intestinal tissues of patients with appendicitis and IBD. Laser capture micro dissection and real time quantitative PCR has been applied to determine the mRNA expression of IL-2. Results: Foxp3+ TR cells accumulate and proliferate in inflamed intestinal tissues. In experimental colitis, the number of contacts between Foxp3+ TR cells and proliferating Ki67+ (Foxp3-) cells significantly exceeded the theoretically expected random distribution. The accumulation of TR cells at proliferating cells is dependent on IL-2. Ki67+ proliferating cells express high level of IL-2 mRNA. Conclusions: Proliferating IL-2 producing cells signal inflammation and provide Foxp3+ TR cells in the intestine with IL-2 that is in turn needed for TR maintenance and immunosuppressive activity.

journal of crohn's and colitis

P298 - Permanent catalase activity impairment due to minor expression of catalase protein in peripheral white mononuclear cells of patients with naive and treated Crohn's disease