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METHODS: Acute DSS colitis was induced in PlGF knock-out (-/-) and wild-type (WT) mice. Disease activity was calculated during the course of the experiment. Mice were sacrificed at several timepoints. Colonic injury was evaluated by colon length, intestinal epithelial apoptosis (TUNEL assay) and histological score. PlGF and VEGF were measured in distal colonic lysates by ELISA. Mucosal vascularization was quantified by computerized morphometric analysis of CD31 stained distal colonic sections. Intestinal hypoxia was visualized by pimonidazole staining and semi-quantitatively analysed by western blot for hypoxia inducible factor alpha (HIF-1α).

P290 - Absence of placental growth factor aggravates DSS-induced acute colonic injury