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The design of a fully automated high-throughput system for the purification of sequencing templates is described. Hardware, software, and chemistries have been optimized to suit the needs of high-throughput laboratories involved in genome sequencing projects. Using this system, up to 5760 samples (60 times 96-well plates) can be purified in less than 16 hours during a single unattended run. The system can also be configured to perform sequencing reaction setup for all 60 plates following template purification, extending total run times to &lt; 24 hours. Final sequencing reactions are prepared in 384-well microplates.

A Fully Automated High-Throughput Nucleic Acid Purification System Using Silica Coated Magnetic Bead Technology