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i) blood cultures: Gram-positive 51%, Gram-negative 44%, mixed 5%, fungi 0%; ii) Septifast: Gram-positive 27%, Gram-negative 59%, mixed 8%, fungi 6%. Using blood cultures as reference, sensitivity of Septifast PCR was 38% (13% in Gram-positive, 57% in Gramnegative), specificity 64%, PPV 26%, NPV 76%. 39% of pathogens not detected by Septifast were not included in the test spectrum. Performance of blood cultures and Septifast PCR in different settings are shown in the table. Microbiological documentation was obtained by Septifast PCR in 29/98 (30%) blood culturenegative episodes: 19/51 (37%) CDI and 10/44 (23%) FUO. 19/23 (83%) pathogens identified by Septifast in CDI were consistent with the site of infection. Fungi were detected in 5 blood culturesnegative episodes of probable or possible invasive mycoses (Candida 4, Aspergillus 1).

Neutropenic Infections in Haematological Malignancies in Greece-: Preliminary Results