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Acoustic abundance of fish depends directly on the target strength (TS) of the fish surveyed. We analyzed 70 and 120 kHz acoustic data from two lakes with abundant rainbow-smelt (Osmerus mordax) populations. Using repeated surveys through the summer growing season, we derived a relationship between TS (dB) and fish length L (cm) at 120 kHz (TS = 19.9 log10 L − 67.8). Values for 70 kHz were similar. In situ TS increased with fish density, indicating a bias from accepting multiple targets at high fish densities. Correcting for this bias increased estimates of smelt abundance by up to 18% in Lake Erie and up to 100% in Lake Champlain. Multiple modes in the TS distributions observed for older fish do not reflect different size groups, as the same modes can be observed from measurements from a single fish. Smelt released gas bubbles during the evening ascent, and these bubbles had TS (−60 to −58 dB) within the range of TS observed from the fish. Gas-bubble release occurred mostly during the migration. Conducting surveys after the ascent is completed will decrease bias associated with counting bubbles as fish.

Application of in situ target-strength estimations in lakes: examples from rainbow-smelt surveys in Lakes Erie and Champlain