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Ultrathin carbon nanomembranes (CNM) comprising crosslinked biphenylprecursors have been tested as support films for energy-filtered transmissionelectron microscopy (EFTEM) of biological specimens. Due to their hightransparency CNM are ideal substrates for electron energy loss spectroscopy(EELS) and electron spectroscopic imaging (ESI) of stained and unstainedbiological samples. Virtually background-free elemental maps of tobacco mosaicvirus (TMV) and ferritin have been obtained from samples supported by ~ 1 nmthin CNM. Furthermore, we have tested conductive carbon nanomembranes (cCNM)comprising nanocrystalline graphene, obtained by thermal treatment of CNM, assupports for cryoEM of ice-embedded biological samples. We imaged ice-embeddedTMV on cCNM and compared the results with images of ice-embedded TMV onconventional carbon film (CC), thus analyzing the gain in contrast for TMV oncCNM in a quantitative manner. In addition we have developed a method for thepreparation of vitrified specimens, suspended over the holes of a conventionalholey carbon film, while backed by ultrathin cCNM.

Energy-filtered transmission electron microscopy of biological samples on highly transparent carbon nanomembranes