English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Aplicação de teste molecular para detecção de adenovírus em pacientes pediátricos distintos The evolution of the methodologies used in the diagnosis of acute respiratory infections of viral etiology is due to the advancement of molecular biology, immunology, genetics and biotechnology. The development of the in vitro cell culture 1 resulted in the isolation of viruses associated with acute respiratory infection of the respiratory tract (respiratory syn-cytial virus, adenovirus and parainfluenza virus). The discovery of the molecular structure of nucleic acids 2 contributed to the development of molecular genetics, responsible, among other things, for the improvement of molecular diagnosis of infectious diseases in the late twentieth century, as well as the disclosure of new viral etiologies related to acute respiratory infection in the first decades of the 21st century. By the end of the 1980s, the diagnosis of respiratory infection consisted in the isolation of the virus in cell cultures and serology. The great challenge faced by scientists at the time was to enhance diagnostic methodologies, aiming to address the appropriate strategies for disease prevention and control; i.e., to establish methodologies that would allow attaining fast and specific diagnoses. 3 Considering the impact of infection by respiratory viruses in all age groups, especially among children <5 years of age, patients with chronic diseases and adults ≥60 years of age, 3,4 a rapid diagnosis is important for: antiviral use; respiratory disease etiology clarification; considering prescription of antibiotics; knowing the natural history of the virus and its physiopathology, which helps to understand the possible complications that may occur depending on the infection characteristics; assessment of disease containment through quarantine; measuring the hospitalization period; preventing unnecessary laboratory investigations, and dispensing with inappropriate isolation of non-infected individuals. The development of monoclonal antibodies in the 1970s 7 contributed to the development of a specific antibody panel for influenza virus types A and B, RSV, ADV, and PIV 1, 2 3, which allows the fast and specific identification by direct or indirect immunofluorescence of the seven respiratory viruses. The advent of molecular methodologies, starting in the 1980s, 8 of which principle resides on the Polymerase Chain Reaction (PCR), allowing the amplification of conserved genome targets of different etiological agents, revolutionized the understanding of epidemiology, diagnosis and research of infectious diseases of viral etiol-ogy, among other approaches. Currently, there are several