English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

SummaryA novel third generation biosensor was developed based on one-shot adsorption of chemically modified cytochrome c (cyt c) onto bare gold electrodes. The introduction of short-chain thiol derivatives (mercaptopropionic acid, MPA) on the lysine residues of cyt c enabled the very fast formation (<5 min) of an electroactive biological self-assembled monolayer (SAM) exhibiting a quasi-reversible electrochemical behavior and a fast direct electron transfer (ET). The high value estimated for the heterogeneous ET rate constant, ks = 1600 s-1, indicates that short anchors might facilitate the ET via an efficient orientation of the heme pocket. In comparison, no direct ET was observed in the case of native and long-anchor modified (mercaptoundecanoic acid, MUA) cyt c adsorbed on gold. The so-made amperometric biosensor enabled real-time and non-invasive detection of extracellular H2O2 released by unicellular aquatic microorganisms Chlamydomonas reinhardtii as a consequence of cadmium-induced oxidative stress

Direct Anchoring of Cytochrome c onto Bare Gold Electrode for Sensing Oxidative Stress in Aquatic Cells