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A complete miniaturized instrument for chip-based capillary gel electrophoresis of DNA with fluorometric detection method and temperature control technique is presented. Separation of single-stranded and double-stranded DNA is obtained in a few minutes utilizing reusable glass chip. Real-time detection is performed using laser-induced-fluorescence and miniature CCD camera. Applied temperature control technique affects mobility of different DNA fragments in polymer sieving matrix. Electrokinetic sample plug injection and separation, temperature stabilization and fluorometric detection are automatically performed in the miniature docking station, controlled by dedicated software with real-time fluorescence signal recognition and digital data conditioning. Presented instrument is automatic, low-cost and portable solution, enabling future application of multitemperature single strand conformation polymorphism (MSSCP) genotyping technique for sensitive detection of minor genetic variants in DNA samples

Miniature instrument for lab-on-a-chip capillary gel electrophoresis of DNA utilizing temperature control technique