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Alcalase endo-protease enzyme was selected in this experiment to extract oil from pine kernel. The response surface analysis method was employed to optimize the parameters in the experiment. The optimum parameters are as follows: enzyme additive amount 1.97%, hydrolysis time 3.0h, hydrolysis temperature 51°C, materials to water rate 1:5, pH 8.4 and total oil extraction rate 89.12%. Factor contribution rate obtained from the F-test are as follows: x2>x1>x4>x3>x5. The fatty acid contents of pine kernel oil are as follows: palmitc acid 3.89%, oleic acid 19.44%, linoleic acid 50.09%, linolenic acid 0.58% and stearic acid 1.53%

Optimization of the aqueous enzymatic extraction of pine kernel oil by response surface methodology