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Dithiobis(succinimidyl propionate) (DTSP) self-assembled monolayer (SAM) functionalized gold microelectrode array has been used for covalent immobilization of monoclonal cortisol antibody (C-Mab). Binding interactions of cortisol to EA/CMab/DTSP/Au electrode was tested using impedimetric technique. This Bio-electrode was used to estimate the concentration of cortisol in samples of saliva and Interstitial Fluid (ISF). Sensors were exposed to solutions with different cortisol concentration and label-free electrochemical impedance (EIS) technique was used to make cortisol measurements. EIS results (change in charge transfer resistance, Rct) confirmed that EA/C-Mab/DTSP/Au based biosensor can accurately detect cortisol in the range of 1 pM to 1 μM has exhibit detection limit of 1 pM. In-vitro measurement of cortisol levels in saliva and ISF shows good correlation with the values obtained with commercial ELISA experiments

Antibody modified gold micro array electrode based electrochemical immunosensor for ultrasensitive detection of cortisol in saliva and ISF