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We have successfully developed an electrochemical biosensor which could detect low level concentration of misty lactic-acid (LA) directly. In our method, enzyme lactate oxidase (LOD) was immobilized within mesoporous silica (FSM8.0) which was formed on screen-printed Prussian Blue (PB)-modified working electrode and the top of that was covered with hydrophilic porous membrane. The resulting biosensor showed an excellent sensitivity (150nM-1.1mM in solution) and can detect misty LA directly. This result indicates that our method provides a high-performance biosensor to determine LA levels in exhaled breath and that our approach is ideal for various medical applications and might improve the detection or management of disease

An Electrochemical Biosensor for the Determination of Lactic Acid in Expiration