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We had combined the Neukirch-Marko model that describes the extension, torqueand supercoiling in single, stretched and twisted DNA of infinite contourlength, with a form of the free energy suggested by Sinha and Samuels todescribe short DNA, with contour length only a few times the persistencelength. We find that the free energy of the stretched but untwisted DNA, issignificantly modified from its infinitely length value and this in turnmodifies significantly the torque and supercoiling. We show that this isconsistent with short DNA being more flexible than infinitely long DNA. We hopeour results will stimulate experimental investigation of torque andsupercoiling in short DNA.

Stretching, Twisting and Supercoiling in Short, Single DNA Molecules