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Isolation of cationic fraction (CF) was achieved by solid phase extraction using an Oasis® MCX cartridge on urine specimens of IC patients and controls. C18 reverse phase high performance liquid chromatography (RP-HPLC) was used to profile cationic metabolites, which were quantified by area under the peaks and normalized to creatinine. Major CF peaks were identified by RP-HPLC and liquid chromatography-tandem mass spectrometry. HTB-4 urothelial cells were used to determine the cytotoxicity of CF and of individual metabolites.

MP39-19 THE ROLE OF URINARY CATIONS IN THE ETIOLOGY OF INTERSTITIAL CYSTITIS: A MULTISITE STUDY