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A POCl3 catalyzed, efficient, one-step and solvent-free synthesis of novel thieno[2,3-d]pyrimidin-4(3H)-one derivatives from 2-amino-4,5-substitutedthiophene-3-carbonitrile has been developed under conventional heating and microwave irradiation. The formation of compounds was confirmed using elemental analysis and spectroscopic techniques like IR, NMR (1H and 13C) and mass spectroscopy. Furthermore, they were screened in vitro to study their antimicrobial activity, which shows weak to moderate activity against all tested microorganisms

POCl 3 catalyzed, one-step, solvent-free synthesis of some novel thieno[2,3- d ]pyrimidin-4(3 H )-one derivatives as antimicrobial agent