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s 219 with increasing Mg content. The Young’s modulus of PLGA/TCP/Mg scaffolds (Mg content: 15% wt) was around 104 Mpa, statistically significantly stronger than that of Mg content: 10% wt (83Mpa) and Mg content 5% wt group (82 Mpa), as well as PLGA/Mg group, 66Mpa. All the Mg containing scaffolds were statistically significantly stronger than PLGA/TCP and PLGA group, 45 Mpa and 30 Mpa, respectively. Conclusion: The results of CCK-8 assay demonstrated the MC3T3-E1 osteoblasts grew very well and proliferated rapidly on PLGA/TCP/Mg scaffolds compared to PLGA/TCP scaffold after 7 day culture. The in vitro study also demonstrated a good biocompatibility and bioactivity of the PLGA/ TCP/Mg scaffold that was in favor of accelerating and inducing the proliferation and differentiation of osteoblasts. Acknowledgements This project was supported by a NSFC grant (512111203 and 51203178), European Union’s 7th Framework Programme (NMP-2013-EU-China proposal, project No. 604517), and SFRF(JCYJ20120617114912864) IBDW2014-00085-F0017 SYNERGISTIC EFFECT OF DECELLULARIZED ANNULUS FIBROSUS (AF) MATRIX AND SUBSTRATE STIFFNESS ON THE GENE EXPRESSION OF RABBIT AF STEM CELLS Jun Li , Chen Liu , Huilin Yang , Bin Li b Department of Orthopaedics, Xiamen Hospital of Traditional Chinese Medicine, China Department of Orthopaedics, The First Affiliated Hospital of Soochow

Synergistic effect of decellularized annulus fibrosus (AF) matrix and substrate stiffness on the gene expression of rabbit AF stem cells