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We describe three new automated methods for purifying genomic DNA from whole blood. The MagneSil ®  Blood Genomic, Max Yield System uses MagneSil ®  paramagnetic particles (PMPs) in a 96-well format to purify the maximal amount of DNA from a 200-μL blood sample. In contrast, the MagneSil ®  ONE, Fixed Yield Blood Genomic System uses MagneSil ®  Fixed Yield PMPs to purify a normalized amount of DNA from 60 μL of blood in a 96-well format. These methods are implemented on the Beckman Coulter Biomek ®  FX automated workstation. The MagneSil ®  KF Genomic System uses MagneSil ®  PMPs to purify DNA from 1 to 15 samples of 200-μL blood using the moderate-throughput Thermo Electron KingFisher ®  mL instrument. The MagneSil ®  Blood Genomic System typically yields &gt; 4 μg per 200 μL of whole blood, depending on the white blood cell content. The MagneSil ®  ONE System is best suited where there is a requirement for purification of a narrow concentration range of DNA. This system purifies 1 μg(±50%) of DNA from 60 μL of blood. The MagneSil ®  KF System purifies 2 to 6 μg of DNA from 200 μL of blood. DNA purified using all of these methods is suitable for PCR, STR, EADIT ®  SNP genotype analysis, and multiplexed PC analysis.

Purifying Genomic DNA from Whole Blood on Automated, High-Throughput, and Moderate-Throughput Platforms