Open AccessIncreasing SARS-CoV-2 RT-qPCR testing capacity by sample pooling
Author(s) -
Julia Alcoba-Flórez,
Helena Gil-Campesino,
Diego García-Martínez de Artola,
Oscar Díez-Gil,
Agustı́n Valenzuela-Fernández,
Rafaela GonzálezMontelongo,
Laura Ciuffreda,
Carlos Flores
Publication year - 2020
Publication title -
international journal of infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.278
H-Index - 89
eISSN - 1878-3511
pISSN - 1201-9712
DOI - 10.1016/j.ijid.2020.11.155
Subject(s) - covid-19 , pooling , real time polymerase chain reaction , outbreak , reverse transcription polymerase chain reaction , medicine , biology , virology , disease , computer science , gene , messenger rna , infectious disease (medical specialty) , genetics , artificial intelligence
Limited testing capacity has characterized the ongoing coronavirus disease 2019 (COVID-19) pandemic in Spain, hampering timely control of outbreaks and opportunities to reduce the escalation of community transmission. This study investigated the potential to use sample pooling, followed by one-step retrotranscription and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to increase testing capacity for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
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