Application of single-strand conformational polymorphism for detection of amino acid changes in neutralizing epitope of hemagglutinin gene of influenza A strains

Background: Chronic hepatitis B (CHB) is a serious health problemworldwidewitha substantialminorityofpatients experiencing premature death due to end-stage liver disease and/or hepatocelluar carcinoma. The purpose of the present study is to clarify whether the changes of liver histology in patients with mild CHB, whose alanine aminotransferase (ALT) is more than and less than two times up limit of normal (ULN), are different. The study will provide clue to solve vital problems in CHB treatment. It is meaning for the cases which even thoughmeet the requirements of both the “Chronic Hepatitis B Prophylaxis and Treatment Guide 2010” and HBV DNA detective value demond for normative anti-virus treatment, but ALT 0.05). However, for staging liver fibrosis, it shows an obvious difference as group ALT≥80U/L holds a higher S1 ratio (P <0.05). It means group ALT≥80U/L bears lower hepatic fibrosis. Conclusion: Since the present study shows that the comparison of hepatic inflammation activity is statistically meaningless comparing across groups of different ALT, and comparing to group ALT≥80U/L, group ALT<80U/L bears more serious hepatic fibrosis. It suggest that for mild CHB patients, includes both ALT≥80u and ALT<80u patients, as long as patients meet HBV DNA viral load demond for normative anti-virus treatment, normative antivirus treatment should be conducted. This study supplements and improves “Chronic Hepatitis B Prophylaxis and Treatment Guide” regarding to antiviral treatment for mild CHB patients with serum ALT abnormal (ALT<2×ULN).