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An efficient agar plate mutagenesis and screening technique for improving mutation frequency was established by using dielectric barrier discharge (DBD) plasma. Enterobacter agglomerans is well known as a phosphate-solubilizing plant-associated bacterium. In this study, DBD plasma was conducted toE. agglomerans mutation for improving the phosphate-soubilizing activity. The results showed that the phosphate-soubilizing activity of mutants increased compared with original strain, and the phosphate-soubilizing activity of the best mutants is 1.49-fold of original strain. It demonstrated that DBD plasma treatment has a high-efficient quality, and it will be a useful method of mutation

Mutation Induction by DBD Plasma in Phosphate-solubilizing Bacteria Enterobacter Agglomerans