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In this work we report on the simultaneous measurement of the hydrodynamiccoefficient and the electric charge of single {\it Bacillus subtilis} spores.The latter has great importance in protein binding to spores and in theadhesion of spores onto surfaces. The charge and the hydrodynamic coefficientwere measured by an accurate procedure based on the analysis of the motion ofsingle spores confined by an optical trap. The technique has been validatedusing charged spherical polystyrene beads. The excellent agreement of ourresults with the expected values demonstrates the quality of our procedure. Wemeasured the charge of spores of {\it B. subtilis} purified from a wild typestrain and from two isogenic mutants characterized by an altered spore surface.Our technique is able to discriminate the three spore types used, by theircharge and by their hydrodynamic coefficient which is related to thehydrophobic properties of the spore surface.

Surface charge and hydrodynamic coefficient measurements of {\it Bacillus subtilis} spore by Optical Tweezers