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We present investigations on volume regulation and beading shape transitionsin PC12 neurites conducted using a flow-chamber technique. By disrupting thecell cytoskeleton with specific drugs we investigate the role of its individualcomponents in the volume regulation response. We find that microtubuledisruption increases both swelling rate and maximum volume attained, but doesnot affect the ability of the neurite to recover its initial volume. Inaddition, investigation of axonal beading --also known as pearlinginstability-- provides additional clues on the mechanical state of the neurite.We conclude that the initial swelling phase is mechanically slowed down bymicrotubules, while the volume recovery is driven by passive diffusion ofosmolites. Our experiments provide a framework to investigate the role ofcytoskeletal mechanics in volume homeostasis.

The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites