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he article on the evaluation of the effectiveness of mature ed cell and reticulocyte parameters under three conditions: ron deficiency anemia, anemia of chronic disease (ACD), nd anemia of chronic disease associated with absolute iron eficiency by Torino et al.1 is very valuable. Automated reticlocyte counts are widely used in the clinical laboratory due o their greater precision, accuracy and reproducibility comared to those obtained using the microscope. The most mportant benefit of automated methods is the greater preision of counts. By analyzing a much greater number of eticulocytes (more than ten thousand), the statistical error s minimized.2 Visual microscopy is still recommended as he comparability method for reticulocytes, despite studes showing that the variation coefficient (VC) ranges from 0% to 40%. Fully automated methods have eliminated nter-observer variability and subjectivity and substantially educed turnaround time. Automated methods employ a ide variety of reagents for reticulocyte RNA and these how different sensitivity on binding to RNA.3 Therefore, lthough automated flow cytometric analysis has led to a ignificant advance in reticulocyte counting, some limitaions still persist in comparability across different laboratories

Comment on: Evaluation of erythrocyte and reticulocyte parameters as indicative of iron deficiency in patients with anemia of chronic disease