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Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyzean enzymatic reaction which carries out the AND logic gate. This logic functionis considered here in the context of biocatalytic processes utilized for thebiocomputing applications for "digital" (threshold) sensing/actuation. Weoutline the response functions desirable for such applications and report thefirst experimental realization of a sigmoid-shape response in one of theinputs. A kinetic model is developed and utilized to evaluate the extent towhich the experimentally realized gate is close to optimal.

biosensors and bioelectronics

Enzymatic AND-Gate Based on Electrode-Immobilized Glucose-6-Phosphate  Dehydrogenase: Towards Digital Biosensors and Biochemical Logic Systems with  Low Noise