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The Staphylococcus aureus enterotoxin type D was cloned and expressed in Escherichia coli X1776 either as mature toxin or as a fusion with E. coli beta-galactosidase. Regulated expression was obtained and levels of toxin produced were in the order of 10(-3)-fold higher than in S. aureus.

Expression of Staphylococcus aureus enterotoxin type D in Escherichia coli X1776