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A colony-immunoblotting procedure is described which allows a quick screening of high numbers of bacteria for their Enterobacterial Common Antigen phenotype. In this assay the intensity of reaction is dependent on the carrier to which ECA is linked and which anchors ECA in the outer membrane. Bacteria containing LPS-linked ECA react stronger in this and in other immunoassays than bacteria containing only the phospholipid-linked ECA.

Detection of enterobacterial common antigen on bacterial cell surfaces by colony-immunoblotting: effect of its linkage to lipopolysaccharide