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HighlightsA framework nucleic acid delivery system was developed through self-assembly, which can deliver antisense oligonucleotides against multiple targets in bacterial cells.    The ASOs-tFNAs (750 nM) was found to simultaneously inhibit the expression of  gtfBCD ,  gbpB , and  ftf , and significantly reduce the extracellular polysaccharide synthesis and biofilm thickness.

Multi-targeted Antisense Oligonucleotide Delivery by a Framework Nucleic Acid for Inhibiting Biofilm Formation and Virulence