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HighlightsVapor nanobubble (VNB) photoporation represents a promising physical technique for mRNA transfection of adherent and suspension cells.    A multitude of parameters related to the VNB photoporation procedure were optimized to enable efficient mRNA transfection.    VNB photoporation was found to yield five times more living, transfected Jurkat T cells as compared to electroporation, i.e., currently the standard nonviral transfection technique for T cells.Electronic supplementary material  The online version of this article (10.1007/s40820-020-00523-0) contains supplementary material, which is available to authorized users.

Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation