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Catharanthus roseus leaves and roots hyper-accumulate the terpenoid indole alkaloids, which are required for the economic production of pharmaceutical molecules vindoline (V), catharanthine (C), vinblastine (VB), vincristine (VC), ajmalicine (A) and serpentine (S). For developing marker-assisted selection (MAS) breeding in C. roseus, the objectives of the present study were: (a) To characterize variability and associations between six traits—V (VL), C (CL), VB + VC (VBL) contents of leaves and C (CR), A (AR) and S (SR) contents in roots, in a recombinant inbred line (RIL) population in two environments. (b) To identify and place quantitative trait loci (QTLs) associated with the traits on the map. The population of 191 RILs was found to segregate transgressively for all the six traits. The intra-organ correlations of leaf and root traits were positive and significant. The inter-organ traits were not correlated. All the traits except AR were highly heritable (h 2 = 41−79 %). A RIL population genetic map of 179 markers on 8 linkage groups (LGs) covering 1,786.5 cM genetic distance was constructed. The RIL-generated genetic map led to identification of 20 QTLs for 6 traits (5, 4, 1, 4, 2, and 4 QTLs, respectively for CL, VL, VBL, CR, AR, and SR) by the use of single marker regression, simple interval mapping and composite interval mapping approaches. QTLs were detected only on 4 of the 8 LGs. QTL for the same or different traits were mapped in clusters or singularly. The results suggest that a combination of MAS and phenotypic selection offer possibilities of realizing simultaneous improvement in C and V accumulation in leaves and roots of C. roseus.

Detection and Mapping of QTLs Affecting Contents of Pharmaceutical Alkaloids in Leaf and Root of Catharanthus roseus