English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Protocatechuate 3,4-dioxygenase was isolated and characterized from Pseudomonas pseudoalcaligenes KF707 for the purpose of developing a new anti-browning agent. The protocatechuate 3,4-dioxygenase from Pseudomonas pseudoalcaligenes KF707 was purified 296.8-fold, and showed specific activity of 121.7 U/mg. Based on the SDS-polyacrylamide and gel permeation chromatography, the molecular weight of protocatechuate 3,4-dioxygenase was 189.9 kDa and was composed of 3 αβ protomers, with molecular weights of 29.0 kDa of α subunit and 34.3 kDa of â subunit. The optimal pH and temperature were 7.5 and 38°C, respectively. Km values of catechol, protocatechuate, gallate, p-cresol, caffeic acid, catechin, L-DOPA, 4-methylcatechol and pyrogallol were 14, 17, 2, 10, 12, 20, 30, 21 and 3 μM, and the Vmax/Km (mim−1) values were 0.052, 3.06, 0.35, 0.01, 0.03, 0.02, 0.006, 0.008 and 0.11, respectively. This indicates that the enzyme is active on a wide range of phenyl compounds, in contrast to the high specificity of similar enzymes from other sources. Our data also show that the turnover number of protocatechuate 3,4-dioxygenase from Pseudomonas pseudoalcaligenes KF707 is 68 s−1, which is much higher than the known values from other sources.

Purification and characterization of protocatechuate 3,4-dioxygenase from Pseudomonas pseudoalcaligenes KF707