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Bioactive constructs to guide cellular mobilizationand function have been proposed as an approach fora new generation of biomaterials in functional tissueengineering. Adult mesenchymal stem cells have beenwidely used as a source for cell based therapeutic strategies,namely tissue engineering. This is a heterogeneouscell population containing many subpopulations with distinctregenerative capacity. Thus, one of the issues for theeffective clinical use of stem cells in tissue engineering isthe isolation of a highly purified, expandable specificsubpopulation of stem cells. Antibody functionalized biomaterialscould be promising candidates to isolate andrecruit specific cell types. Here we propose a new conceptof instructive biomaterials that are able to recruit andpurify specific cell types from a mixed cell population.This biomimetic concept uses a target-specific chitosansubstrate to capture specific adipose derived stem cells.Specific antibodies were covalently immobilized ontochitosan membranes using bis[sulfosuccinimidyl] suberate(BS3). Quartz crystal microbalance (QCM) was used tomonitor antibody immobilization/adsorption onto thechitosan films. Specific antibodies covalently immobilized kept their bioactivity and captured specific cell types froma mixed cell population. Microcontact printing allowed tocovalently immobilize antibodies in patterns and simultaneouslya spatial control in cell attachmentThe authors acknowledge the Portuguese Foundation for Science and Technology (FCT) for the fellowships SFRH/BD/61390/2009 (C.A.C) and SFRH/BPD/45206/2008 (A.M.F), and also to the International Max-Planck-Research School (C.A.C) for the financial support. We are grateful to Hospital da Prelada for the donations. This work was carried out under the scope of the EU 7th Framework Programme (FP7/2007-2013) under grant agreement no. NMP4-SL-2009-229292 (Find&Bind)

Selective Cell Recruitment and Spatially Controlled Cell Attachment on Instructive Chitosan Surfaces Functionalized with Antibodies