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Two polyene macrolide antibiotics: antifungalmycin ( 1 ) and fungichromin ( 2 ) were isolated from the culture mycelia of  Streptomyces padanus  702 via bioactivity-guided fractionation using various chromatographic procedures. Their structures were elucidated on the basis of spectral analysis, and  1  is a new polyene macrolide. Compounds  1  and  2  showed significant inhibition against  Gibberella zeae  with EC 50  values of 26.71 and 2.21 µg/mL,  Fusicoccum  sp. (plantain head blight) with EC 50  values of 23.4 and 3.17 µg/mL,  Mucor  ssp. 8894 with EC 50  values of 28.80 and 2.11 µg/mL,  Ustilaginoidea virens  with EC 50  values of 26.72 and 0.21 µg/mL, respectively. This shows that the microbial secondary metabolites  1  and  2  have the potential to be developed as agricultural fungicides for use against  G. zeae, Fusicoccum  sp.,  Mucor  ssp. 8894, and  U. virens .Electronic Supplementary Material  Supplementary material is available for this article at 10.1007/s13659-011-0037-1 and is accessible for authorized users.

Antifungalmycin, an antifungal macrolide from Streptomyces padanus 702