Open AccessEfficient generation of mouse ESCs-like pig induced pluripotent stem cells
Author(s) -
Qi Gu,
Jie Hao,
Tang Hai,
Jianyu Wang,
Yundan Jia,
Qingran Kong,
Juan Wang,
Chunjing Feng,
Binghua Xue,
Bingteng Xie,
Shichao Liu,
Jingyu Li,
Yilong He,
Jialu Sun,
Lei Liu,
Liu Wang,
Liu Z,
Qi Zhou
Publication year - 2014
Publication title -
protein and cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.973
H-Index - 63
eISSN - 1674-8018
pISSN - 1674-800X
DOI - 10.1007/s13238-014-0043-2
Subject(s) - induced pluripotent stem cell , developmental biology , stem cell , embryonic stem cell , biology , microbiology and biotechnology , genetics , gene
Dear Editor, Porcine induced pluripotency stem cells (piPSCs) are promised in basic research, animal husbandry and regenerative medicine. However, the efficiency of the piPSCs induction has been low and the generated piPSCs varied in cell morphology and cell characteristics. Here we report a novel approach to improve efficiency of piPSCs generation. The induced piPSCs are dome-shaped mouse embryonic stem cells (ESCs)-like and display molecular properties of mouse ESCs. Electroporation study reveals that mouse ESCslike status facilitates genetic manipulating of piPSCs. Importantly, we demonstrate that the domed piPSC colonies are more suitable as donor cells for nuclear transfer (NT) to generate reconstructed embryos than those flattened piPSCs. The potential applications of the newly generated piPSCs in ungulate pluripotent research are discussed.
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