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Numerous new taxa and classifications of Dothideomycetes have been published following the last monograph of families of Dothideomycetes in 2013. A recent publication by Honsanan et al. in 2020 expanded information of families in Dothideomycetidae and Pleosporomycetidae with modern classifications. In this paper, we provide a refined updated document on orders and families  incertae sedis  of Dothideomycetes. Each family is provided with an updated description, notes, including figures to represent the morphology, a list of accepted genera, and economic and ecological significances. We also provide phylogenetic trees for each order. In this study, 31 orders which consist 50 families are assigned as orders  incertae sedis  in Dothideomycetes, and 41 families are treated as families  incertae sedis  due to lack of molecular or morphological evidence. The new order, Catinellales, and four new families,  Catinellaceae ,  Morenoinaceae Neobuelliellaceae  and  Thyrinulaceae  are introduced. Seven genera ( Neobuelliella ,  Pseudomicrothyrium ,  Flagellostrigula ,  Swinscowia ,  Macroconstrictolumina ,  Pseudobogoriella , and  Schummia ) are introduced. Seven new species ( Acrospermum urticae ,  Bogoriella complexoluminata ,  Dothiorella ostryae ,  Dyfrolomyces distoseptatus ,  Macroconstrictolumina megalateralis ,  Patellaria microspora , and  Pseudomicrothyrium thailandicum ) are introduced base on morphology and phylogeny, together with two new records/reports and five new collections from different families. Ninety new combinations are also provided in this paper.

Refined families of Dothideomycetes: orders and families incertae sedis in Dothideomycetes