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Neochloris minuta  and  Neochloris alveolaris  grown in nitrogen-rich (+ N) and nitrogen-depleted (-N) media were tested for their heavy metal maximum biosorption capacities (q max ) and adsorption percent efficiencies (R%). By removing nitrogen from the growth media, both algal species showed an increase in their lipid content and a decrease in their protein content. Langmuir and Freundlich adsorption isotherms were used to determine the q max  and adsorption efficiencies of the + N and −N algae in the recovery of Pb 2+ , Cd 2+ , Zn 2+ , Cu 2+ , and Ni 2+ . When comparing the two types of algae,  N. alveolaris  showed the highest adsorption capacities for all five metals either in + N or -N media. The maximum adsorption efficiency percentage of the lowest concentration metal ions for  N. alveolaris  was 87.10% for Pb 2+ , 64.98% for Cd 2+ , 59.50% for Zn 2+ , 60.08% for Cu 2+ , and 50.61% for Ni 2+ . In both algae, nitrogen depletion (-N) caused an increase in the q max  values for Zn 2+  and Cu 2+ . Additionally, the q max  of  N. minuta  for Cd 2+ , Zn 2+ , Cu 2+  and Ni 2+  increased by the nitrogen depletion demonstrating that the treatment can be applied to improve the biosorption capacity of a particular alga for multiple heavy metals. The biosorption capacity for these algae for heavy metals was also discussed in terms of their biomass compositions and the type of hard or soft metal acid based on the Pearson theory of Hard and Soft, Acid and Bases (HSAB).

Effects of nitrogen depletion on the biosorption capacities of Neochloris minuta and Neochloris alveolaris for five heavy metals