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The giant water bug ( Abedus herberti ) is a large flightless insect that is a keystone predator in aridland aquatic habitats. Extended droughts, possibly due to climate change and groundwater pumping, are causing once-perennial aquatic habitats to dry, resulting in serious conservation concern for some populations. A. herberti also exhibits exclusive male parental care, which has made it a model organism for studying mating systems evolution. Here we describe 17 novel polymorphic microsatellite loci developed for A. herberti . Number of alleles per locus ranged from 2 to 15, and average observed and expected heterozygosities were 0.579 and 0.697, respectively. These loci can successfully resolve both population genetic structure among sites separated by 3-100 km ( F  ST = 0.08-0.21, P &lt; 0.0001), and divergent mating strategies within local populations, making them highly useful for conservation genetics studies of this vulnerable species.

17 novel polymorphic microsatellite markers for the giant water bug, Abedus herberti (Belostomatidae)