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The most important parameter to obtain an appropriate preservation condition of museum environments concerns the indoor air quality. The exposure of artwork and materials to gaseous and particulate pollutants introduced by visitors and either indoor or outdoor sources contributes to their decay. In this work, we evaluated the possible monitoring of the visitors’ influence using the stable carbon isotopic ratio of CO 2  and the concentration of NH 3  as a real-time tool. The study was done in the Refectory of Santa Maria delle Grazie (Milan, Italy) which houses one of the most important paintings of Leonardo da Vinci, the Last Supper, and had more than 400,000 visitors in 2019. The results confirmed a good correlation between the presence of tourists inside the museum and the variation of δ 13 C value during the visits and the closure of the museum. The variation of indoor atmospheric δ 13 C was influenced by the presence of visitors in the Refectory and delineates the way done from the entrance to the exit. In the same way, the concentration of NH 3  was influenced by the presence of visitors and confirmed the role of this one on preservation methodology for indoor air quality in the museum. This new methodology can be used as a supplemental and non-invasive tool to help in calibrating microclimatic conditions through the ventilation rate and air filtration systems in the museum and to manage the number of visitors per turn.

Leonardo da Vinci’s “Last Supper”: a case study to evaluate the influence of visitors on the Museum preservation systems