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Recent studies in this laboratory showed that an extracellular cutinase from  A. mediterranei  (AmCut) was able to degrade the plastics polycaprolactone and polybutylene succinate. Such plastics can be slow to degrade in soils due to a lack of efficient polyester degrading organisms. AmCut also showed potential for the biocatalytic synthesis of esters by reverse hydrolysis. The gene for AmCut has an upstream leader sequence whose transcript is not present in the purified enzyme. In this study, we show using predictive modelling, that this sequence codes for an N-terminal signal peptide that directs transmembrane expression via the Sec secretion pathway.  E. coli  is a useful host for recombinant enzymes used in biocatalysis due to the ease of genetic manipulation in this organism, which allows tuning of enzymes for specific applications, by mutagenesis. When a truncated GST-tagged AmCut gene (lacking its signal peptide) was expressed in  E. coli , all cutinase activity was observed in the cytosolic fraction. However, when GST-tagged AmCut was expressed in  E. coli  along with its native signal peptide, cutinase activity was observed in both the periplasmic space and the culture medium. This finding revealed that the native signal peptide of a Gram-positive organism (AmCut) was being recognised by the Gram-negative ( E. coli ) Sec transmembrane transport system. AmCut was transported into  E. coli’ s periplasmic space from where it was released into the culture medium. Surprisingly, the presence of a bulky GST tag at the N-terminus of the signal peptide did not hinder transmembrane targeting. Although the periplasmic targeting was unexpected, it is not unprecedented due to the conservation of the Sec pathway across species. It was more surprising that AmCut was secreted from the periplasmic space into the culture medium. This suggests that extracellular AmCut translocation across the  E. coli  outer membrane may involve non-classical secretion pathways. This tuneable recombinant  E. coli  expressing extracellular AmCut may be useful for degradation of polyester substrates in the environment; this and other applications are discussed. Graphical abstract

world journal of microbiology and biotechnology incorporating the mircen journal of applied microbiology and biotechnology/world journal of microbiology and biotechnology

Extracellular secretion of a cutinase with polyester-degrading potential by E. coli using a novel signal peptide from Amycolatopsis mediterranei