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Commercial probiotics preparation containing  Bacillus coagulans  have been sold in the market for several decades. Due to its high intra-species genomic diversity, it is very likely that  B. coagulans  strain may alter in different ways over multiple years of production. Therefore, the present study focuses to evaluate the genetic consistency and probiotic potential of  B. coagulans  MTCC 5856. Phenotypic and genotypic techniques including biochemical profiling, 16S rRNA sequencing, GTG 5″, BOX PCR fingerprinting, and Multi-Locus-Sequence typing (MLST) were carried out to evaluate the identity and consistency of the  B. coagulans  MTCC 5856. Further, in vitro probiotic potential, safety and stability at ambient temperature conditions of  B. coagulans  MTCC 5856 were evaluated. All the samples were identified as  B. coagulans  by biochemical profiling and 16S rRNA sequencing. GTG 5″, BOX PCR fingerprints and MLST studies revealed that the same strain was present over 3 years of commercial production.  B. coagulans  MTCC 5856 showed resistance to gastric acid, bile salt and exhibited antimicrobial activity in in-vitro studies. Additionally,  B. coagulans  MTCC 5856 was found to be non-mutagenic, non-cytotoxic, negative for enterotoxin genes and stable at ambient temperature (25 ± 2 °C) for 36 months. The data of the study verified that the same strain of  B. coagulans  MTCC 5856 was present in commercial preparation over multiple years of production.   Electronic supplementary material  The online version of this article (doi:10.1007/s11274-016-2027-2) contains supplementary material, which is available to authorized users.

world journal of microbiology and biotechnology incorporating the mircen journal of applied microbiology and biotechnology/world journal of microbiology and biotechnology

Evaluation of genetic and phenotypic consistency of Bacillus coagulans MTCC 5856: a commercial probiotic strain