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The effects of phosphate processing wastewater (PPWW) on heavy metal accumulation in a Mediterranean soil (Tunisia, North Africa) were investigated. Moreover, the residual toxicities of PPWW-irrigated soils extracts were assessed. Results showed that heavy metal accumulation was significantly higher in PPWW-irrigated soil extracts than in control soil. The heavy metal accumulation increased over time in treated soil samples and their average values followed the following order: Iron (Fe 252.72 mg l −1 ) > Zinc (Zn 152.95 mg l −1 ) > Lead (Pb 128.35 mg l −1 ) > Copper (Cu 116.82 mg l −1 ) > Cadmium (Cd 58.03 mg l −1 ). The residual microtoxicity and phytotoxicity of the various treated soil samples extracts were evaluated by monitoring the bioluminescence inhibition (BI %) of  Vibrio ficheri  and the measurement of the germination indexes (GI %) of  Lepidium sativum  and  Medicago sativa  seeds. The results showed an important increase of residual toxicities of PPWW-treated soil extracts over time.

Study of Heavy Metal Accumulation and Residual Toxicity in Soil Saturated with Phosphate Processing Wastewater