English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

A high yield of isolated protoplasts and efficient regeneration protocols are prerequisites for successful development of somatic hybrids. In the present study, protoplast isolation and regeneration were evaluated in 12 Kalanchoë accessions belonging to nine species. The highest protoplast yield was obtained from K. blossfeldiana ‘Charming Red Meadow’ with 10.78 ± 0.51 × 105 protoplasts per gram fresh weight. We observed significant differences of protoplast yield while there was no distinct difference in viability among the accessions. Seven accessions reached the microcolony stage and four developed microcalli in medium supplemented with 1.0 mg/l 1-naphthaleneacetic acid (NAA), 0.5 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D). Using five selected accessions we optimized the PGR (plant growth regulators) concentrations using combinations of NAA, BAP and 2,4-D. K. blossfeldiana cultivars ‘Charming Red Meadow’ and ‘Paris’ produced significantly different numbers of calli depending on the PGR concentrations. For plant regeneration, the medium was supplemented with 1 mg/l NAA and 2 mg/l BAP or 2 mg/l zeatin. Shoots were regenerated on medium supplemented with NAA and BAP for K. blossfeldiana ‘Charming Red Meadow’ and K. blossfeldiana ‘Paris’. The plants successfully developed roots on the medium supplemented with IAA. The medium containing zeatin induced root formation directly from callus in K. blossfeldiana ‘Charming Red Meadow’. Our findings have the potential to facilitate the use of Kalanchoë species in somatic hybridization breeding programs.Key MessageThe study revealed a strong genotype-dependent efficiency of colony and microcallus formation. Plants were regenerated from two Kalanchoë blossfeldiana cultivars on medium supplemented with NAA and BAP.

Protoplast isolation and culture from Kalanchoë species: optimization of plant growth regulator concentration for efficient callus production