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Leaf chlorophyll (Chl) concentration can be an indicator of plant health, including photosynthetic potential and nutrient status. In some cases, this measure can indicate the degree to which plants are water-stressed. Traditional methods of measuring Chl concentration have involved a destructive sampling technique: extraction and spectrophotometric analysis. A compatible nondestructive method to measure leaf Chl concentration exists and applies transmittance spectroscopy to plants with a  meter. These techniques were evaluated by comparing leaf Chl concentration in big bluestem (). Leaves were sampled from plants representing three ecotypes (originating from Central Kansas, Eastern Kansas, and Illinois, USA) and two cultivars of  growing in Hays, Kansas, USA. Leaf Chl concentration was measured using nondestructive and destructive techniques. We documented a saturating relationship between destructively measured leaf Chl concentration and SPAD index resulting from a decelerating change in SPAD index as Chl concentration increased. The comparison of  ecotypes and cultivars demonstrated highest Chl concentration in the ecotype and cultivar from areas with historically low precipitation, Central Kansas and  var. , respectively. A high ratio of Chl  to Chl  is an index of drought adaptation and was also manifested in  from drier regions. Thus, drought-adapted ecotypes and cultivars might be able to maintain high photosynthetic productivity and protect photosystem II during dry periods. Conversely, the ecotypes and cultivar originating from areas with higher precipitation had lower leaf Chl and a lower Chl  ratio.

photosynthetica

A comparison of seasonal foliar chlorophyll change among ecotypes and cultivars of Andropogon gerardii (Poaceae) by using nondestructive and destructive methods