English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Particle estimation is a classical problem arising in many science fields, such as biophysics, fluid mechanics and biomedical imaging. Many interesting applications in these areas involve 3D imaging data: This work presents a technique to estimate the 3D coordinates of the center of spherical particles. This procedure has its core in the processing of the images of the scanned volume: It firstly applies denoising techniques to each frame of the scanned volume and then provides an estimation of both the center and the profile of the 2D intersections of the particles with the frames, by coupling the usage of Total Variation functional and of a regularized weighted Least Squares fit. Then, the 2D information is used to retrieve the 3D coordinates using geometrical properties. The experiments provide evidence that image denoising has a large impact on the performance of the particle tracking procedures, since they strongly depend on the quality of the initial acquisition. This work shows that the choice of tailored image denoising technique for Poisson noise leads to a better estimation of the particle positions.

Efficient Position Estimation of 3D Fluorescent Spherical Beads in Confocal Microscopy via Poisson Denoising