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The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different tissues were detected by quantitative real-time PCR (qPCR). The results showed that the mRNA expressions of GAD 65 , GAD 67 , and GABA-T in the telencephalon (Tel), mesencephalon (Mes), cerebella (Cer), and medulla oblongata (Med) were downregulated significantly with the safe dose (SD, 20 mg/kg) of DIF (P &lt; 0.05 or P &lt; 0.01). While the expressions of GAD 65 and GAD 67 in the kidney at 12 h had strikingly upregulated to 13.81 ± 1.06** and 150.67 ± 12.85** times. Treated with the lethal dose of 50% (LD 50 , 2840 mg/kg b. W.) of DIF, the mRNA expressions of GAD 65 , GAD 67 , and GABA-T in all tissues were increased significantly (P &lt; 0.01). The results of AVM group showed that the mRNA expressions of GAD 65 , GAD 67 , and GABA-T both in the central and peripheral tissues were all remarkably downregulated at the safe concentration (SC, 0.0039 mg/L) and the lethal concentration of 50% (LC 50 , 0.039 mg/L), except for the mRNA inhibitions of GAD 65 , GAD 67 , and GABA-T in the muscle at 2 h which sharply downregulated to 0.20 ± 0.02 ΔΔ  × 10 -2 , 0.57 ± 0.06 ΔΔ  × 10 -1 and 0.44 ± 0.02 ΔΔ  × 10 -1 , respectively (P &lt; 0.01).

Effects of two kinds of fishery drugs on the expressions of GAD and GABA-T mRNA in crucian carp (Carassius auratus gibelio)