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Cerebral glycogen is principally localized in astrocytes rather than in neurons. Glycogen metabolism has been implicated in higher brain functions, including learning and memory, yet the distribution patterns of glycogen in different types of astrocytes have not been fully described. Here, we applied a method based on the incorporation of 2-NBDG, a D-glucose fluorescent derivative that can trace glycogen, to investigate glycogen's distribution in the brain. We identified two types of astrocytes, namely, 2-NBDG I (glycogen-deficient) and 2-NBDG II (glycogen-rich) cells. Whole-cell patch-clamp and fluorescence-activated cell sorting (FACS) were used to separate 2-NBDG II astrocytes from 2-NBDG I astrocytes. The expression levels of glycogen metabolic enzymes were analyzed in 2-NBDG I and 2-NBDG II astrocytes. We found unique glycogen metabolic patterns between 2-NBDG I and 2-NBDG II astrocytes. We also observed that 2-NBDG II astrocytes were mainly identified as fibrous astrocytes but not protoplasmic astrocytes. Our data reveal cell type-dependent glycogen distribution and metabolism patterns, suggesting diverse functions of these different astrocytes.

Single-Cell Analysis for Glycogen Localization and Metabolism in Cultured Astrocytes