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This study describes a multi-wavelength spectroscopic method for the determination of carboxyl group content of nanocellulose. Methylene blue (MB) was used as a color indicator, which can bind to the carboxyl groups in nanocellulose to form a R-COOMB complex with a binding ratio of 1:1, although the spectrums of MB and R-COOMB at 500–750 nm are overlapped, and solid impurities could cause spectral interference. To solve these issues, a multi-wavelength spectroscopic measurement on a MB and nanocellulose mixture solution technique and mathematical model were developed, allowing the content of carboxyl groups in nanocellulose to be quantified. The results showed that the method has good measurement precision and accuracy as compared with those measured by a reference method. The method is simple and requires only a small sample size for testing and thus makes it suitable for nanocellulose related research and applications.

A simple multi‐wavelength spectroscopic method for the determination of carboxyl group content in nanocellulose