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Morphometric analysis of nuclei is crucial in cytological examinations. Unfortunately, nuclei segmentation presents many challenges because they usually create complex clusters in cytological samples. To deal with this problem, we are proposing an approach, which combines convolutional neural network and watershed transform to segment nuclei in cytological images of breast cancer. The method initially is preprocessing images using color deconvolution to highlight hematoxylin-stained objects (nuclei). Next, convolutional neural network is applied to perform semantic segmentation of preprocessed image. It finds nuclei areas, cytoplasm areas, edges of nuclei, and background. All connected components in the binary mask of nuclei are treated as potential nuclei. However, some objects actually are clusters of overlapping nuclei. They are detected by their outlying values of morphometric features. Then an attempt is made to separate them using the seeded watershed segmentation. If the attempt is successful, they are included in the nuclei set. The accuracy of this approach is evaluated with the help of referenced, manually segmented images. The degree of matching between reference nuclei and discovered objects is measured with the help of Jaccard distance and Hausdorff distance. As part of the study, we verified how the use of a convolutional neural network instead of the intensity thresholding to generate a topographical map for the watershed improves segmentation outcomes. Our results show that convolutional neural network outperforms Otsu thresholding and adaptive thresholding in most cases, especially in scenarios with many overlapping nuclei.

Cell Nuclei Segmentation in Cytological Images Using Convolutional Neural Network and Seeded Watershed Algorithm