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An immunomagnetic separation method using antibody-coated Dynabeads ® Protein G was developed for specific and efficient separation of Staphylococcus aureus in lettuce and whole milk. The amount of immunomagnetic beads (IMBs) and conjugation conditions were optimized. A high capture efficiency was obtained with 0.4 mg of IMBs, an immunoreaction time of 20 min, and a separation time of 1 min without wash. Under optimal conditions, the capture efficiency (CE) for 10 0 -10 5 CFU/mL of S. aureus was higher than 91.46%. The IMBs showed high specificity even with a high constant number (10 7 CFU/mL) of Bacillus cereus , Micrococcus luteus , and Lactobacillus plantarum . The CE of IMBs against S. aureus at concentrations from 10 2 to 10 5 CFU/mL ranged from 78.70 to 94.77% for lettuce and 60.0 to 73.27% for milk samples. This IMS can be an appropriate selection for combining with bacterial detection method or efficient isolation procedure for S. aureus from foods.

Highly efficient and specific separation of Staphylococcus aureus from lettuce and milk using Dynabeads protein G conjugates